tb-500 mastery course
Unit 9 of 12

dosing & administration

community-reported protocols, reconstitution, stacking, and cycling -- for educational purposes only

The Dosing Landscape

TB-500 runs on a two-phase model: a 4-6 week loading phase at mg doses twice weekly, then a reduced maintenance phase. The cadence tracks TB4's long tissue half-life -- plasma clears rapidly while tissue concentrations remain elevated for days, explaining why twice-weekly injections are the most widely reported community protocol.

Human PK is thin. Ruff 2010 tolerated IV TB4 up to 1,260 mg, but a Phase I trial says little about injury-repair dosing. Protocols below come from animal work and user reports.

2x/wk
typical loading cadence
4-6 wk
loading phase length
1,260 mg
max IV dose tolerated (Ruff 2010)
0
human trials in injury healing

Educational only, not medical advice. No regimen here is validated in human injury-healing trials.


Run the Dosing Numbers

pick a vial size and protocol; see reconstitution and weekly totals.

dosing reference calculator

Why Twice-Weekly? The Half-Life Story

plasma clears in hours; tissue holds for days. step through the PK timeline.

TB4 pharmacokinetics timeline

loading phase vs maintenance phase

the two-phase protocol explained side by side.

loading phase

  • duration: 4-6 weeks
  • frequency: twice weekly
  • community dose range: 2-5 mg per injection
  • goal: saturate tissue compartments and establish a working concentration
  • evidence basis: extrapolated from animal weight-based dosing -- no human data

maintenance phase

  • duration: variable, often 4-8 weeks or as needed
  • frequency: once weekly or less
  • community dose range: 1-2 mg per injection
  • goal: sustain tissue levels at a lower running cost
  • evidence basis: purely community-derived; no clinical pharmacology supports this phase
educational only. these protocols are community-reported and not validated in human clinical trials. not medical advice.

key terms

pharmacokinetics — half-life

time for plasma concentration to drop by 50%. TB-500's plasma half-life is short (minutes to hours) but tissue concentrations remain elevated for days, explaining the twice-weekly injection schedule.

pharmacokinetics — reconstitution

dissolving a lyophilized (freeze-dried) peptide vial in bacteriostatic water to create an injectable solution. concentration depends on vial size and water volume added.

dosing protocol — loading phase

an initial higher-dose period (e.g. 2x/week for 4-6 weeks) intended to build up tissue levels before transitioning to maintenance dosing. not validated in human trials.

dosing protocol — maintenance phase

ongoing lower-dose injections (e.g. 2 mg every 1-2 weeks) used after a loading period to maintain the hypothesized therapeutic effect. entirely community-derived; no clinical basis.